HISTOCHEMICAL DEMONSTRATION OF 3α-HYDROXYSTEROID DEHYDROGENASE ACTIVITY

The Histochemical Demonstration of Glyceraldehyde-3-phosphate Dehydrogenase Activity

A histochemical method for demonstration of glyceraldehyde-3-phosphate dehydrogenation by tissues is described. The method utilizes Nitro BT as an indicator, glyceraldehyde-3-phosphate obtained from hydrolysis of commercially obtainable glyceraldehyde-3-phosphate diethylacetal (monobarium salt) as substrate, and (ethylenediamine)tetraacetic acid acid disodium as an activating agent in a medium ...

Activation of human liver 3α-hydroxysteroid dehydrogenase by sulphobromophthalein

Human liver contains at least two isoenzymes (DD2 and DD4) of 3α-hydroxysteroid}dihydrodiol dehydrogenase. The NADP(H)-linked oxidoreductase activities of DD4 were activated more than 4-fold by sulphobromophthalein at concentrations above 20 μM and under physiological pH conditions. Sulphobromophthalein did not stimulate the activities of DD2 and human liver aldehyde reductase, which are functi...

Histochemical Demonstration of Dehydrogenase Activity in the Cells of Normal Human Blood and Bone Marrow

Endogenous and succinic dehydrogenase activity was demonstrated in the living cells of normal human blood and bone marrow using a buffered nitro BT-succinate incubating solution. With this technique dehydrogenase activity was localized primarily in the granular leukocytes and the sites of enzymatic activity appeared to be non-mitochondrial. The addition of a non-ionic surface active agent to th...

Histochemical Demonstration of the Sites of Activity of Dehydrogenase Systems with the Electron Microscope

In the present study a histochemical method demonstrating the activity of dehydrogenase systems was developed for electron microscopy, utilizing potassium tellurite as the hydrogen or electron acceptor. This reagent was used intravitally (intravenously, intraperitoneally, or intraluminally in hollow organs) or supravitally on small blocks of tissue for the demonstration of endogenous dehydrogen...

Simplified procedure for the histochemical demonstration of dehydrogenase activity in rat ovaries.